北京小龙门东方中杜鹃多重寄生冕柳莺的报道

The Multiple Brood Parasitism of Eastern Crowned Warbler by Oriental Cuckoo in Xiaolongmen National Forest Park of Beijing

多重巢寄生是1只或多只寄生性鸟类在1个宿主巢内产2枚或多枚卵的特殊行为方式。对于雏鸟具有排他性的杜鹃而言,多重寄生被认为是种内个体之间或种间的一种竞争,但相关报道较少。通过野外观察和分子生物学检测技术,我们在北京小龙门国家森林公园确定了一个被东方中杜鹃(Cuculus optatus)多重寄生的冕柳莺(Phylloscopus coronatus)巢。

Using field observations and molecular analysis,we found a case of multiple brood parasitism（ two parasitic eggs in one host nest） in Xiaolongmen National Forest Park,Beijing,China. The host was the Eastern Crowned Warbler（ Phylloscopus coronatus）,and the two parasitic eggs belonged to the Oriental Cuckoo（ Cuculus optatus）. One parasitic egg was successfully hatched,the other failed. We took blood sample from the hatched nestling and host bird,and tissue sample from two eggs that were out of the nest. DNA was extracted from these samples and the Cyt b gene were amplified via the polymerase chain reaction（ PCR） and compared with Cyt b gene sequences in GenBank. The results indicated that both the hatched nestling and failed parasitic egg belonged to the Oriental Cuckoo. However,we can not recognized whether the two parasitic eggs came from one or two birds of Oriental Cuckoo.