摘要
目的探讨准分子激光原位角膜磨镶术(LASIK)后1年内基质金属蛋白酶-9(MMP-9)在人眼泪液中的动态变化。方法收集无明显并发症的32例LASIK患者术前及术后7、14 d和1、2、3、6、12月的泪液,应用免疫印迹技术检测MMP-9的浓度变化。结果 LASIK术后泪液中MMP-9的浓度呈动态变化,且与术后时间的长短密切相关。术后14 d泪液中MMP-9浓度达到高峰,约为术前的2.7倍;随后逐渐下降,但在术后12个月MMP-9仍未恢复术前水平,约为术前的1.38倍。结论 LASIK术后泪液中MMP-9的浓度呈时间依赖性变化,术后14 d达到高峰,随后下降,但术后1年仍未恢复至术前水平,提示LASIK术后的角膜创伤修复反应至少持续1年。
Objective To investigate the dynamic changes of matrix metalloproteinase-9 (MMP-9) level in tear fluid within 12 months after laser in situ keratomileusis (LASIK). Methods Twenty-two myopic patients undergoing uneventful LASIK were enrolled in this study. Tear fluid samples were collected from the patients for measurements of MMP-9 level using Western blotting preoperatively, at 7 and 14 days, and at 1, 2, 3, 6, and 12 months after the surgery. Results MMP-9 concentrations in the tear fluid of post-LASIK patients showed a time-dependent variation pattern. MMP-9 reached its peak level in the tear fluid at 14 days postoperatively, which was 2.70 times the preoperative level; it gradually decreased thereafter but was still 1.38 times the preoperative level at 12 months after the surgery. Conclusions MMP-9 concentrations in the tear fluid of post-LASIK patients show a time-dependent variation pattern and remains higher than the preoperative level even at 12 months after the surgery, suggesting that corneal wound healing after LASIK lasts for more than 12 months.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2014年第8期1079-1082,共4页
Journal of Southern Medical University
基金
Supported by grants from the Science and Technology Council of Guizhou Province([2011]025,[2008]118)~~