准分子激光原位角膜磨镶术后1年内泪液中基质金属蛋白酶-9的动态变化(英文)

Dynamic changes of tear fluid matrix metralloproteinase-9 within 1 year after laser in situ keratomileusis

目的探讨准分子激光原位角膜磨镶术(LASIK)后1年内基质金属蛋白酶-9(MMP-9)在人眼泪液中的动态变化。方法收集无明显并发症的32例LASIK患者术前及术后7、14 d和1、2、3、6、12月的泪液,应用免疫印迹技术检测MMP-9的浓度变化。结果 LASIK术后泪液中MMP-9的浓度呈动态变化,且与术后时间的长短密切相关。术后14 d泪液中MMP-9浓度达到高峰,约为术前的2.7倍;随后逐渐下降,但在术后12个月MMP-9仍未恢复术前水平,约为术前的1.38倍。结论 LASIK术后泪液中MMP-9的浓度呈时间依赖性变化,术后14 d达到高峰,随后下降,但术后1年仍未恢复至术前水平,提示LASIK术后的角膜创伤修复反应至少持续1年。

Objective To investigate the dynamic changes of matrix metalloproteinase-9 （MMP-9） level in tear fluid within 12 months after laser in situ keratomileusis （LASIK）. Methods Twenty-two myopic patients undergoing uneventful LASIK were enrolled in this study. Tear fluid samples were collected from the patients for measurements of MMP-9 level using Western blotting preoperatively, at 7 and 14 days, and at 1, 2, 3, 6, and 12 months after the surgery. Results MMP-9 concentrations in the tear fluid of post-LASIK patients showed a time-dependent variation pattern. MMP-9 reached its peak level in the tear fluid at 14 days postoperatively, which was 2.70 times the preoperative level; it gradually decreased thereafter but was still 1.38 times the preoperative level at 12 months after the surgery. Conclusions MMP-9 concentrations in the tear fluid of post-LASIK patients show a time-dependent variation pattern and remains higher than the preoperative level even at 12 months after the surgery, suggesting that corneal wound healing after LASIK lasts for more than 12 months.