不同输液方案对兔肠缺血再灌注损伤小肠细胞凋亡的影响

The effect of different transfusion protocals on intestinal apoptosis in rabbit intestinal ischemia-reperfusion injury

目的观察限制性输液和开放性输液对兔肠缺血再灌注损伤小肠细胞凋亡的影响。方法 70只日本大白耳兔随机分成3组,对照组(control,n=10)、肠缺血再灌注损伤(IRI)限制性输液组(RFG,n=30)、肠IRI开放性输液组(LFG,n=30),然后于肠IRI后24、48和72 h各处死10只(n=10),取小肠回盲部用TUNEL法检测小肠细胞凋亡,用qRT-PCR检测其凋亡基因Bcl-2和Bax在mRNA水平上的表达。结果兔肠缺血再灌注后3个时间点RFG小肠细胞的凋亡数明显少于LFG(P<0.01)。Bcl-2的表达水平RFG明显高于LFG(P<0.01),而促凋亡基因Bax的表达低于LFG(P<0.01)。结论与开放性输液方案相比,限制性输液方案能减轻兔肠缺血再灌注损伤,减少小肠细胞凋亡。

Objective To investigate the effect of different transfusion protocals （restrictive fluid and liberal fluid） on intestinal apoptosis in rabbit intestinal ischemia-reperfusion injury （IRI）. Methods Seventy male rabbits were randomly allocated into three groups, normal control group （NCG, n = 10 ） , intestinal IRI restrictive fluid group （ RFG, n = 30）, intestinal IRI liberal fluid group （ LFG, n = 30）, 24, 48 and 72 h after intestine IRI separately rabbits were killed （n = 10）. Intestine was cut to detect intestinal apoptosis by TUNEL and the expression of Bcl- 2, Bax by q-RT-PCR were detected. Results The number of intestinal apoptosis after intestinal IRI in restrictive fluid group was significantly lower than that in liberal fluid group （ P 〈 0. 01 ）. Anti-apoptosis gene Bel-2 in RFG was higher than that in LFG （ P 〈 0. 01 ）, while proapoptosis gene Bax in RFG was less than LFG （ P 〈 0. 01 ）. Conclusions The effect restrictive fluid on intestine is mild than LFG, which may be potentically explained by apoptosis of much less.