HPV感染、HIF-1α及VEGF蛋白表达与非小细胞肺癌相关

Infection of HPV and its relationship with the expression of HIF-1α and VEGF protein in non-small cell lung cancer

目的探讨人乳头状瘤病毒(HPV)感染在非小细胞肺癌(NSCLC)发生中的病因学意义,并初步分析HPV感染与低氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)蛋白表达之间的关系。方法用免疫组化法检测60例NSCLC及20例肺良性病变组织中HIF-1α和VEGF蛋白表达,用PCR法选用HPV16、18型特异性引物检测两组中HPV DNA的表达。结果 1)NSCLC组HPV DNA积分吸光度比值为0.6046±0.0224,显著高于肺良性病变组的0.0275±0.1230(P<0.05)。2)NSCLC组HIF-1α和VEGF蛋白的平均吸光度值分别为0.2548±0.0219和0.2051±0.0321,均显著高于肺良性病变组(0.0826±0.0150和0.0726±0.0227)(P<0.05)。3)NSCLC中HIF-1α的表达率与VEGF呈正相关(P<0.05)。4)NSCLC中HPV(+)组VEGF的表达率为56.0%,显著高于HPV(-)组的28.6%(P<0.05)。结论 HPV感染可能是NSCLC发生的病因学因素之一;HPV感染可能通过上调VEGF的表达,促进肺癌的发生发展。

Objective To investigate the etiological role of human papillomavirus （HPV） infection in the carcinogenesis of non-small cell lung cancer（NSCLC） and to discuss the possible relationship between HPV infection and HIF-1 α, VEGF expression. Methods By using immunohistochemistry, the expressions of HIF-1α and VEGF protein were detected in 60 cases of NSCLC tissues and 20 cases of lung benign diseases tissues. PCR was employed to detect the expression of HPV16/18 DNA. Results 1 ） The HPV DNA expression was 0. 6046 ±0. 0224 in NSCLC group which significantly higher than 0. 0275±0. 1230 in the benign diseases group （P 〈0. 05）. 2）The HIF-1α protein and VEGF protein expressions were 0. 2548 ±0. 0219 and 0. 2051 ±0. 0321 respectively in NSCLC, significantly higher than that in the benign diseases group （0. 0826±0. 0150 and 0. 0726 ±0. 0227） （P 〈0. 05）. 3）The expression rate of HIF-1α was significantly correlated with VEGF in NSCLC （P 〈 0. 05 ）. 4）The positive expression rate of VEGF was significantly higher in HPV DNA positive group （56. 0% ） than that in HPV DNA negative group（28. 6%,P 〈 0. 05） in NSCLC. Conclusions HPV infection may be one of the etiological factors in the carcinogenesis of NSCLC. HPV infection increases VEGF expression, promotes the occurrence and development of NSCLC.