盐蒿内生耐(嗜)盐真菌的分离及鉴定

Isolation and identification of endophytic halotolerant and halophilic fungi associated with Artemisia halodendron

采用含盐浓度不同的培养基分离陕北花马盐湖盐蒿(Artemisia halodendron)的内生耐(嗜)盐真菌并研究其物种多样性。从盐蒿组织中共分离得到1 215株内生耐(嗜)盐真菌,基于形态学和ITS系统发育分析表明,上述菌株属于6个属,包括小球腔霉属(Leptosphaeria)、青霉属(Penicillium)、木霉属(Trichoderma)、曲霉属(Aspergillus)、链格孢属(Alternaria)和镰孢属(Fusarium)。以小球腔霉属(Leptosphaeria)为优势属,其次为链格孢属,其分离频率分别为78.8%和2.28%;共计9个代表种如产黄青霉(Penicillium chrysogenum)、Aspergillus westerdijkiae、极细链格孢(Alternaria tenuissima)和长梗木霉(Trichoderma longibrachiatum)等。研究表明,陕北花马盐湖盐蒿中蕴藏着丰富的耐(嗜)盐真菌物种资源。

This study isolated and identified species diversity of fungal endophytes in Artemisia halodendron using different media complemented with the different concentration of NaCl. We have obtained 1215 fun-gal isolates from A. halodendron,and these fungi are classified into 6 genera including Leptosphaeria,Penicil-lium,Trichoderma,Aspergillus,Alternaria and Fusarium. Leptosphaeria is predominant（78. 8% ）of the total number,followed by Alternaria（2. 28% ）. All the fungal strains are identified as 9 species such as Penicilli-um chrysogenum,Aspergillus westerdijkiae,Alternaria tenuissima and Trichoderma longibrachiatum. Our study shows that halotolerant and halophilic fungi in A. halodendron grown in the salt Huama lake in Northern Shaanxi are rich.