菊花品种的遗传多样性分析及CDDP指纹图谱构建

Genetic diversity and construction of fingerprinting of chrysanthemum cultivars by CDDP markers

利用来源保守DNA序列多态性（CDDP）标记技术，探讨50个不同花径、瓣型、花色的菊花品种的遗传多样性。筛选的19条CDDP引物，共产生234个条带，平均每条引物产生12．32个条带，其中211个（90．17％）为多态性条带。菊花品种间的SM相似系数变化范围为0．6170～0．9447，平均0．7330。至少需要2条引物组合（WRKY-R2和WRKY-R3）可以完全区分50个品种。UPGMA聚类结果表明，所选的菊花品种基本按花径聚类，与瓣型和花色无直接关系。研究表明CDDP技术可有效用于菊花遗传多样性分析和品种鉴定研究。

This study used conserved DNA-derived polymorphism （CDDP） markers to analyse the genetic diversity among 50 chrysanthemum cuhivars with different inflorescence sizes, petal and colour types. Using 19 selected CDDP primers, 234 bands were generated with an average of 12.32 bands per primer, with 211 （90.17%） being polymorphic. The SM similarity coefficient among different pairs of genotypes varied from 0. 617 0 to 0. 944 7, with an average of 0. 733 0. The minimum number of primers required to distinguish all 50 accessions was 2 （WRKY-R2 and WRKY-R3）. The dendrogram conducted by UPMGA method showed that clustering closely corresponded to inflorescence size and not to petal types or colour types. The results demonstrate that CDDP marker is a very effective and efficient tool for genetic diversity analysis and cultivar identification.