低剂量γ射线照射后的大鼠血清代谢组学研究

Serum metabonomics of rats irradiated by low-dose γ-rays

目的探讨低剂量γ射线对大鼠血清中代谢产物的影响。方法健康雄性SD大鼠16只,随机均分为正常对照组和照射组。照射组经60Coγ射线每天照射1h,照射剂量率为72mGy/h,连续照射7d。实验结束后腹主动脉取血,分离血清,采用核磁共振氢谱(1H-NMR)仪进行检测,获取两组大鼠血清样本代谢指纹图谱,采用主成分分析(PCA)和正交信号校正-偏最小二乘法(OSC-PLS)进行模式识别,并通过SIMCA-P软件鉴别两组代谢物谱的差异。结果1H NMR图谱分析发现对照组和照射组大鼠血清的代谢谱存在明显差异,OSC-PLS分析显示两组代谢产物各自聚集,分布区域明显分开。与对照组比较,照射组血清中脂质、葡萄糖、肌酸、甘氨酸/葡萄糖、高密度脂蛋白、低密度脂蛋白、极低密度脂蛋白/低密度脂蛋白、不饱和脂肪酸含量增加,乳酸、苏氨酸/脂质、丙氨酸、N-乙酰糖蛋白1、N-乙酰糖蛋白2、饱和脂肪酸和磷脂酰胆碱含量降低。结论低剂量γ射线照射可引起大鼠血清代谢产物变化,主要涉及免疫功能、能量代谢、糖代谢和脂代谢。

Objective To explore the effect of low-doseγ -rays on the metabolites in rat serum. Methods Sixteen healthy male SD rats were randomly divided into control group and irradiated group （n=8）. The rats in irradiated group were irradiated by 60Co γ -rays with a dose rate of 72mGy/h for 7 days （1 hour per day）. At the 7th day after irradiation, blood samples were taken from abdominal aorta to obtain the serum. The metabolic fingerprints of serum were obtained from the two groups of rats, and 1H nuclear magnetic resonance （NMR） spectroscopy, principal component analysis （PCA） and orthogonal signal correction-partial least squares （OSC-PLS） method were used for pattern recognition, and the difference in metabolite profile between two groups was identified by SIMCA-P software. Results The rat serum 1H NMR spectra revealed a significant difference between control group and irradiated group, the OSC-PLS plots of the serum samples presented marked clustering between two groups. Compared with the control group, the content of lipid, glucose, creatine, glycine/glucose, high density lipoprotein, low density lipoprotein, very low density lipoprotein/low density lipoprotein and unsaturated fatty acid increased, while the content of lactic acid, threonine/ lipid, alanine, N-acetyl glycoprotein 1, N-acetyl glycoprotein 2, saturated fatty acid and phosphatidyl choline decreased in irradiated group. Conclusion Irradiation with low-dose γ-ray could induce changes in metabolites in rat serum, concerning mainly immune function, energy metabolism, carbohydrate metabolism and lipid metabolism.