慢性缺氧对大鼠行为和海马齿状回DCX阳性细胞的影响

Effects of chronic hypoxia on behavior and DCX positive cells in hippocampal dentate gyrus of rats

目的:研究慢性低压性缺氧对大鼠行为和海马齿状回DCX阳性细胞的影响。方法:建立SD大鼠慢性低压性缺氧模型,通过开场实验和蔗糖饮水实验观察大鼠行为变化。取脑组织冰冻切片,用免疫荧光检测海马齿回神经元前体细胞标志物微管相关蛋白Doublecortin(DCX)检测海马齿状回DCX阳性细胞的变化。结果:与对照比较,慢性低压性缺氧大鼠体重,开场实验水平运动得分,垂直运动得分降低和蔗糖偏嗜度均明显降低(P<0.01)。免疫荧光双标检测显示,慢性低压性缺氧大鼠海马齿状回颗粒下层(SGZ)DCX阳性细胞减少;DCX阳性细胞平均胞体周长(μm):实验组(151.2±16.1)明显大于对照组(132.8±14.5,P<0.01);DCX阳性细胞突起平均长度(μm):实验组(21.7±7.3)明显大于对照组(15.2±5.6,P<0.01)。结论:慢性低压性缺氧大鼠行为学上可发生明显改变,海马齿状回DCX阳性细胞再生能力减弱,提示缺氧导致该细胞再生损害可能是发生行为学改变的基础。

Objective： To study effects of chronic low oxygen on the behavior and hippocampal dentate gyms DCX positive cells in rats. Methods： Chronic low pressure hypoxia model was set up in SD rats, behavior changes were observed by open-field and sucrose preference, and changes of hippocampal dentate gyms DCX positive cells was determined by immunofluorescence staining. Results： Compared with controls, the body weight, the opening experiment horizontal motion score, score lower vertical movement and sucrose partial heavy degrees were significantly lower in chronic low-pressure isehemic rat （P 〈 0.01 ）. Immunofluorescence staining showed that DCX positive cells in dentate gyms subgranular zone （SGZ） decreased, and the mean soma perimeter was significantly higher than that of the control group （132.8 ± 14.5 μm vs 151.2 ± 16.1μm, P 〈 0.01 ）, and the average length of DCX positive cell processes was significantly higher than that of the control group （21.7 ± 7.3 μm vs 15.2 ± 5.6 μm, P 〈 0.01 ） in the experimental group. Conclusion： The regeneration of hippocampal dentate gyms DCX positive cells might decrease at chronic hypoxia condition in rat modie.