龙岩市一起暴发性脑炎疫情分离的埃可病毒6型基因特征分析

Analysis of Genetic Characteristics of ECHO6 Virus Isolated from An Epidemic Outbreak of Encephalitis in Longyan,China

对2010年福建省龙岩市长汀县埃可病毒6型（ECHO6）肠道病毒引起的暴发性脑炎,进行病原学分析,为该病的防控提供有价值的信息。对病例脑脊液标本进行荧光RT-PCR、病毒分离（RD细胞）、中和鉴定进行病原确认,再经PCR法对其中4株毒株进行VP1片段或全基因组核苷酸序列测定,用DNAStar软件中的MegAlign等软件进行同源性分析,Mega 4.0软件进行进化树分析。经病原学检测方法确认为ECHO6型肠道病毒,VP1片段核苷酸序列分析显示为C2亚型。全基因组序列共7 407个核苷酸,与其他ECHO病毒和CVB（Coxsackie virus B）病毒基因组构成相似,同源性为78.5%~87.3%。此次暴发性脑炎是由肠道病毒ECHO6C2亚型引起,病毒株全基因组序列长度与标准株（U16283）相近,同源性达80.4%。

This study aimed to analyze the etiology of the encephalitis outbreak in Longyan,Fujian Province,China in 2010,in order to provide valuable information for this prevention and control of this disease.Pathogens were confirmed from cerebrospinal fluid samples with fluorescent RT-PCR,virus isolation（RD cells）,and neutralization tests.Then,the VP1fragments or whole genome nucleotide sequences were determined for four virus strains using PCR.Homology was assessed using the MegAlign software,and a phylogenetic evolutionary tree was drawn using Mega 4.0software.The results confirmed that the etiology of the outbreak was the ECHO6intestinal virus,and the nucleotide sequence of the VP1segment indicated that the C2subtype was responsible.The genome sequence consisted of 7407nucleotides,and resembled the genome of other ECHO and CoxB viruses with homology levels of 78.5%-87.3%.The encephalitis outbreak in Longyan in 2010was caused by the ECHO6C2subtype intestinal virus,and its complete genome sequence length is similar to the standard strain（U16283）with a sequence homology of80.4%.