环介导等温扩增技术的改进及其在现场检测平台上的应用进展

Advance in Loop- Mediated Isothermal Amplification Technique and Its Applications in Point-of-Care Testing Platforms

环介导等温扩增技术(Loop-mediated isothermal amplification,LAMP)是一种新型核酸体外扩增技术,具有等温、快速、高特异性和灵敏度、产物检测简便等特点。自建立以来,该技术被广泛应用于病原检测、动物胚胎的性别鉴定、转基因食品检测和肿瘤的基因检测等领域并得到不断改进和完善,主要体现在特异性提高,反应速度加快,样本处理简化,检测方法特异化,多重扩增得以实现。由于LAMP满足现场检测的诸多要求,当前基于LAMP技术的现场检测平台正向着微型化、集成化、自动化方向发展,并呈现出与芯片实验室和数字扩增等技术联合应用的趋势。本文综述了近年来LAMP技术的改进及其在现场检测平台上的应用进展。

Loop-mediated isothermal amplification（LAMP）is a novel in vitro nucleic acid amplification method conducted under isothermal conditions with the advantages of high specificity,sensitivity,rapidity and easy detection.Since it was established in 2000,it has been widely applied in various fields of analytical science including the diagnosis of a variety of pathogens,identification of embryo sex,detection of genetically modified organisms and cancer gene identification.Additionally,significant progress has been made in the optimization of the LAMP method,such as accelerated reactions,simplified sample processing,the realization of multiplex amplification,and the enhanced specificity of reaction and detection methods.LAMP technology also shows much potential to be adopted as part of point-of-care testing platforms by the micromation,automation and integration with other technologies such as Lab-on-a-Chip and digital nucleic acid amplification.This review summarizes the latest advances in the LAMP technique and its applications in developing point-of-care testing platforms.