1∶49枸橼酸钠抗凝剂在临床输血检验中的应用

Application of 1∶49 sodium citrate anticoagulant on clinical transfusion

目的:研究1∶49枸橼酸钠抗凝剂在血型鉴定、抗体筛查、临床交叉配血和输血传染性指标检测中的应用。方法:配制不同浓度的枸橼酸钠比例,确定最大抗凝比例,取68例标本分别用EDTA-K2、肝素钠和1∶49枸橼酸钠抗凝,分别用来鉴定血型和做抗体筛查,最后用聚凝胺法及微柱凝胶卡式法交叉配血检测不同抗凝剂对交叉配血的结果的影响。同时,取乙肝表面抗原(HBsAg)、丙肝抗体(HCV)、艾滋病抗体(HIV)、梅毒抗体(TP)各30例阳性标本,采用化学发光方法进行检测,观察1∶49枸橼酸钠抗凝管对检测HBsAg、HCV、HIV、TP的影响。结果:1∶49的枸橼酸钠抗凝管能达到抗凝效果,对血型鉴定、抗体筛查、交叉配血结果没有影响,与不抗凝采血管对HBsAg、HCV、HIV、TP阳性标本进行检测比较,2种采血管对结果的影响差异无统计学意义(P>0.05)。结论:1∶49枸橼酸钠抗凝剂能提高临床交叉配血、输血前检查相关传染性指标检测的快速性和准确性。

Objective：To study the application of 1 ： 49 sodium citrate anticoagulation in blood typing, antibody screening, clinical cross matching and blood transfusion infectious indicator detection. Method： In different concentrations, we determined the largest proportion of sodium citrate anticoagulation. Collecting a specimen of 68 cases with EDTA-K2,heparin sodium and sodium citrate anticoagulation. Respectively, identified the blood type and antibody screening method, finally using poly condensation amine method and micro column gel cassette did cross matching to detect different anticoagulant effects on cross matching results. Taking HBsAg, HCV, HIV and TP each 30 positive specimens, then we observed the effect of largest proportion sodium citrate anticoagulation tubes in these indicators, using chemiluminescence method for testing. Result：With no anticoagulant blood vessels of HBsAg, HCV, HIV and TP positive specimen testing for comparison,1 ： 49 sodium citrate anticoagulation tubes could reach anticoagulant effect. The two types of vessels＇ influence on the results had no statistically significant difference （P〉0.05）. Conclusion：For clinical cross matching and blood transfusion infectious indicator detection, 1 ： 49 sodium citrate anticoagulation can improve its quickness and accuracy of detection.