牛磺酸对氧化应激损伤的神经保护作用

Neuroprotection of taurine induced by hydrogen peroxide

目的:探讨牛磺酸对过氧化氢(H2O2)引起的神经元损伤的保护作用。方法:采用不同浓度的H2O2处理神经元,制备体外氧化应激模型,造模前24 h分别给予5、15、25 mmol/L牛磺酸预处理。采用特异性荧光探针DCFH-DA检测神经元活性氧自由基水平,检测乳酸脱氢酶(LDH)释放率反映神经元损伤程度,用免疫印迹法检测脑源性营养因子(brain-derived neurotrophic factor,BDNF)及突触相关蛋白的表达水平。结果:与Control组比较,不同浓度的H2O2(25、50、100μmol/L)均能使LDH向胞外释放增多(P<0.05),15、25 mmol/L的牛磺酸均能减少H2O2引起的LDH的释放(P<0.05)。100μmol/L H2O2使神经元胞内活性氧水平升高,仅25 mmol/L的牛磺酸能抑制H2O2诱导的胞内活性氧的增多(P<0.05)。100μmol/L H2O2处理神经元导致BDNF、突触相关蛋白Synapsin和Spinophilin表达水平下降,给予25 mmol/L的牛磺酸能部分逆转H2O2诱导的蛋白水平的下降(P<0.05)。结论:牛磺酸能够减轻H2O2引起的神经元损伤,具有较好的神经保护作用。

Objective：To explore the protective effect of taurine on injured neurons induced by hydrogen peroxide（H2O2）.Methods：Neurons were treated with different doses of H2O2.Oxidative stress model was prepared in vitro.Taurine was applied at5,15 or 25 mmol / L 24 h prior to H2O2treatment.Specific fluorescent probe DCFH-DA was performed to analyze reactive oxygen species level of neurons.Lactic dehydrogenase（LDH） release assay was performed to detect the injury degree of neurons.Brainderived neurotrophic factor（BDNF） and synaptic-related protein levels were evaluated by Western blotting.Results：Compared with the control group,different doses of H2O2（5,50 and 100 μmol / L） increased LDH release to the extracellular medium（P〈0.05）,while 15 and 25 mmol / L taurine decreased the release（P〈0.05）.One hundred μmol / L H2O2led to an increase of reactive oxygen species level and decreased BDNF,synapsin and spinophilin protein expressions（P〈0.05）.After treatment with 25 mmol / L taurine,the above indicators were attenuated（P〈0.05）.Conclusion：Taurine can alleviate H2O2induced neuron damages and show neuroprotective effect against oxidative stress.