利用高通量测序快速检测H7N9禽流感病毒及基因组序列分析

Detection of avian influenza A( H7N9) virus by high-throughput sequencing and genomic sequence analysis

目的探索用高通量测序技术检测咽拭子样本中H7N9禽流感病毒的方法。方法分别提取编号为106、089及024 3份样本RNA,反转录,cDNA扩增,用Ion Torrent进行高通量测序,对测序结果进行生物信息学分析。结果 3个咽拭子样本的测序结果中均能检测出禽流感病毒,拼接出的基因组序列可以进行生物信息学分析。结论利用高通量测序技术可以快速地检测H7N9禽流感病毒,并可用生物信息学软件拼接基因组序列及进行序列分析。

Objective To explore the methods of high-throughput sequencing for avian influenza A （H7N9） virus detection from throat swabs.Methods RNA was extracted from three throat swab specimens,followed by reverse transcription,amplification of the cDNA,and then the cDNA amplicons were sequenced with Ion Torrent.Finally the sequencing data were analyzed using bioinformatics software.Results Avian influenza A （H7N9） virus could be detected from throat swab specimens by direct sequencing the cDNA amplicon using next-generation sequencing technique,and the genome sequences could be rapidly determined.Conclusion High-throughput sequencing is a powerful tool for detection of avian influenza A （H7N9） virus and the genome sequences can be analyzed using bioinformatics software.