CXCR4在急性髓系白血病骨髓间充质干细胞内的表达及分布

Expression and distribution of CXCR4 in bone marrow mesenchymal stem cells of acute myeloid leukemia

目的探讨CXCR4在初治急性髓系白血病(AML)患者骨髓间充质干细胞(BM-MSCs)中的分布、表达及其临床意义。方法通过提取初次诊断的AML患者化疗前后骨髓单个核细胞,进行BM-MSCs体外分离与培养;用免疫荧光共聚焦的方法观察各组P2代细胞中CXCR4的亚细胞器定位以及在细胞膜上的分布;采用Western blot法检测各组细胞CXCR4的蛋白表达量的变化。结果在初治组AML患者BM-MSCs内,CXCR4较缓解组及对照组分布异常,未在高尔基体内蓄积,多分布在细胞膜上,并且CXCR4蛋白含量明显低于其他两组。结论初治AML患者BM-MSCs内CXCR4分布和含量改变可能与骨髓造血微环境改变有关,并且对白血病细胞的髓外浸润有协同作用。

Objective To explore the distribution,expression of CXCR4 in bone marrow mesenchymal stem cells （BM-MSCs） of untreated acute myeloid leukemia （AML） patients and its clinical significance.Methods Bone marrow samples from AML patients with initial diagnosis who were treated before and after chemotherapy,then isolated and cultured BM-MSCs in vitro.Subcellular localization and distribution of CXCR4 on membrane in P2 cells of different groups were observed by using confocal immunofluorescence.Content changes of CXCR4 were detected by using Western blot.Results The distribution of CXCR4 in BM-MSCs of untreated AML group was different from others,did not accumulate in the Golgi apparatus,most of which were located on the cell membrane instead,and protein content was lower than the other two groups.Conclusion The distribution and content change of CXCR4 in BM-MSCs of untreated AML patients may be associated with the change of bone marrow hematopoietic microenvironment,and CXCR4 has a synergistic effect on extramedullary infiltration of leukemic cells.