摘要
目的 了解脑胶质瘤中ppENK、EDNRB和CLDN-7 3种基因的甲基化状况.方法 应用巢式聚合链式反应（nPCR）方法和亚硫酸氢盐修饰后测序（BSP）方法,分别检测46例脑胶质瘤标本和10例正常脑组织标本的3种基因启动子区CpG岛的甲基化状态,并分析各基因甲基化程度与临床资料的关系.结果 正常脑组织标本中该3种基因无甲基化.脑胶质瘤中ppENK、EDNRB和CLDN-7 3种基因启动子甲基化率分别为44.2％（19/43）、22.5％ （9/40）和0％ （0/5）.在低级别（Ⅰ～Ⅱ级）脑胶质瘤组中ppENK基因启动子甲基化率（33.3％）低于高级别（Ⅲ～Ⅳ级）脑胶质瘤组中ppENK基因启动子甲基化率（69.2％）,两者差异具有统计学意义（P＜0.05）.结论 脑胶质瘤中ppENK和EDNRB基因启动子区CpG岛有甲基化现象,与脑胶质瘤发生、发展有关.
Objective To study the methylation of ppENK,EDNRB and CLDN-7 in glioma.Methods nPCR and BSP were adopted to investigate the promoter frequency of these genes in 46 cases of glioma and 10 normal brain tissue.The relationship between frequency of methylation of these genes and clinical disease material was analyzed.Results Normal brain tissue had no genes methylation.In patientions with glioma,the frequency of methylation in ppENK,EDNRB and CLDN-7 was 44.2％ （19/43）,22.5 ％ （9/40） and 0％ （0/5）.The rate （33.3％） of ppENK promoter methylation in grades Ⅰ ～ Ⅱ gliomas was significantly lower than that （69.2％） in grades Ⅲ ～ Ⅳ gliomas （P ＜ 0.05）.Conclusion ppENK and EDNRB promoter （CpG island）hypermethylation exists in glioma,which may correlate with the occurrence of glioma.
出处
《安徽医科大学学报》
CAS
北大核心
2014年第8期1124-1127,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省卫生厅基金(编号:2010B013)
