成年裸鼠和成人体表皮肤的比较组织形态学研究

The histological comparasion of the adult nude-mice skin to the adult human skin

取成年裸鼠及成人体表皮肤,用HE染色、MassonFontana染色及透射电镜等方法,观察二者全层皮肤组织学结构及黑色素分布情况。提示二者皮肤的组织学结构在光镜水平存在差异,裸鼠无汗腺,皮下有横纹肌层;Masson-Fontana染色在裸鼠显示为阴性结果。然而在电镜水平可以发现裸鼠皮肤基底层存在黑色素细胞。

After skin flaps were harvested from the adult nude-mice and the adult human,they were made into sections and stained by HE stain or Masson-Fontana stain.Thus the skin structures and the distribution of melanin were observed under the light microscopy.The ultra-structure of melanocytes was detailed by transmission electromicroscopy.Under the light-microscopic observation,sweat glands were found agenesis in nude mice,with a thin layer of straited muscle shown in the subcutaneous layer.Masson-Fontana stain showed negative results in nude mice,but under transmission electromicroscopy,melanocytes were prominent in the basal layer.