福建地区鼻咽癌与人乳头状瘤病毒感染的相关性

The correlation of human papillomavirus and EB virus infection in nasopharyngeal carcinoma in Fujian

目的:初步探讨中国福建地区鼻咽癌与人乳头状瘤病毒(HPV)感染的相关性。方法:收集经病理诊断明确为鼻咽癌的石蜡标本70例(鼻咽癌组),其中角化型鳞状细胞癌9例,非角化型癌61例(非角化分化型癌31例,非角化未分化型癌30例);以鼻咽部慢性炎症标本25例作为正常对照组。采用表面等离子谐振法(SPR)和聚合酶链反应法(PCR)检测鼻咽癌和正常组织中的HPV DNA;运用免疫组织化学方法检测鼻咽癌和正常组织中的HPV 16/18E6蛋白和EB病毒潜伏膜蛋白-1(LMP-1);运用原位杂交的方法检测EBER。结果:SPR和PCR法检测的70例鼻咽癌标本中有2例HPV DNA阳性,其中1例HPV-18阳性,为非角化未分化型癌;1例HPV-70阳性,为非角化分化型癌。2例HPV阳性标本中未检测出EBV相关指标。鼻咽癌标本中未检出HPV 16/18E6蛋白。70例鼻咽癌病理标本中EB病毒LMP-1的检出率为35.71%(25/70),EBER的检出率为81.43%(57/70)。正常对照组未检出HPV-DNA、HPV 16/18E6蛋白、LMP-1和EBER。结论:中国福建地区鼻咽癌中人乳头状瘤病毒感染率较低,该地区的鼻咽癌中无HPV和EB病毒共同感染现象。

Objective：To investigate the prevalence of human papillomavirus in nasopharyngeal carcinomas of Fujian province in China.Method：Samples from 70patients with NPC and 25controls.All samples were detected HPV DNA by polymerase chain reaction（PCR）suing GP5＋/6＋and MY09/11primers and genotyped by surface plasmon resonance（SPR）and HPV 16/18E6and LMP-1using immunohistochemistry and EBER using in situ hybridization.Result：Only 2cases of 70patients were showed evidence of HPV DNA by PCR,the 2HPV positive cases subtype HPV-70and HPV-18were genotyped by SPR,both the 2HPV positive cases are non-keratinizing carcinomas（the HPV-70positive one is differentiated and the HPV-18positive one is undifferentiated）,both the 2HPV positive cases do not show any evidence of EBV.Data showed that 57of 70NPC detected EBER positive,but only 25out of 70NPC samples were detected LMP-1positive.Conclusion：Our study showed a low prevalence of human papillomavirus in NPC patients of Fujian province in Southern China,there is no evidence about HPV and EBV co-infection.