摘要
目的了解黄芪对1型糖尿病小鼠的治疗作用。方法 48只小鼠随机分为对照组、糖尿病组、小剂量黄芪组、大剂量黄芪组、胰岛素组、胰岛素+黄芪组,每组8只。对照组腹腔注射0.5mL柠檬酸缓冲液,其余5组给予链脲佐菌素(STZ)诱导制备小鼠1型糖尿病模型,给予相应药物治疗4周。ELISA法检测各组小鼠血清一氧化氮(NO)、诱导型一氧化氮合成酶(iNOS)水平,病理切片观察胰岛炎积分。结果大、小剂量黄芪组和黄芪联合胰岛素组血清NO及iNOS水平与糖尿病组比较显著下降(F=12.137、15.628,P<0.05),胰岛素组血清NO及iNOS水平与糖尿病组比较差异无显著性(P>0.05)。实验组胰岛炎积分与对照组比较差异有显著性(H=89.586,P<0.05),但各实验组间比较差异无显著性(P>0.05)。结论黄芪在体内能够显著降低1型糖尿病小鼠iNOS活性,减少NO生成,但是不能有效降低血糖及减轻胰岛炎症。
Objective To investigate the therapeutic effect of Astragalus on type 1 diabetic mice. Methods Fortyeight mice were equally randomized to six groups as: control group, diabetes group, lowdose Astragalus (A) group, high-dose A group, insulin group, and insulin+ A group. The mice in the control group were given intraperitoneal injection of 0.5 mL citric acid buffer solution. The rest were given intraperitoneal injection of STZ to create a model of type 1 diabetes and treated accordingly for four weeks. The levels of serum NO and inducible nitric oxide synthase (iNOS) were detected by enzyme linked immunosorbent assay method,and insulitis score was evaluated pathologically. Results The serum NO and iNOS levels in low-and high-dose A and insulin+A groups were lower than the diabetes group (F= 12.137,15,628;P〈0.05), but the differences of the serum markers between insulin and diabetes groups were not significant (P〉0.05). A comparison between the control group and each experimental group showed significant differences in terms of integration of pancreatitis (H =89.586,P〈0.05), but the differences between each experimental group were not significant (P〉0.05). Conclusion Astragalus can, in vivo, reduce the iNOS activity and NO production in mice, but can not effectively decline blood sugar and relieve insulitis.
出处
《青岛大学医学院学报》
CAS
2014年第5期401-403,407,共4页
Acta Academiae Medicinae Qingdao Universitatis
基金
国家自然基金资助项目(81170762)
关键词
黄芪
糖尿病
l型
一氧化氮
一氧化氮合酶
小鼠
astragalus membranaceus
diabetes mellitus, type 1
nitric oxide
nitric oxide synthase
mouse