miR-129-1-3p在浆液性卵巢癌中的表达及临床意义

Expression and clinical significance of miR- 129- 1- 3p in serous ovarian cancer

目的:检测miR-129-1-3p在浆液性卵巢癌组织与正常输卵管伞端组织中的表达,并分析miR-129-1-3p与卵巢浆液性上皮性癌临床病理特征之间的关系。方法:采用实时荧光定量聚合酶链反应(qRT-PCR)定量分析100例浆液性卵巢上皮性癌组织、50例正常输卵管伞端组织中miR-129-1-3p的表达,并将检测结果与临床和病理特征资料进行统计学分析。结果:浆液性卵巢癌组织中的miR-129-1-3p表达量显著低于正常输卵管伞端组织(P<0.01)。miR-129-1-3p的表达与FIGO分期、患者年龄、病理分化程度、淋巴结转移、血清CA125值无统计学差异(P>0.05)。结论:miR-129-1-3p可能作为抑癌因子参与了卵巢上皮性癌的发生发展,对卵巢上皮性癌具有潜在的辅助诊断意义,为寻找卵巢上皮性癌的生物靶向治疗提供理论依据。

To detect the miR - 129 - 1 - 3p expression in the epithelial ovarian cancer and analyze the correlation of its expression with clinicopathonological features. Methods：Quantitative real - time PCR was employed to test the miR - 129 - 1 - 3p expression in 150 specimens（including 100 malignant serous epithelial ovarian cancer and 50 the normal fimbriated end of the distal fallopian tube）. The relevance between the miR - 129 - 1 - 3p expres-sion and the pathoclinical characteristics was assessed. Results：The expression of miR - 129 - 1 - 3p was significant-ly down - regulated in patients with serous ovarian cancer compared to normal controls（P ﹤ 0. 01）. Low expression of miR - 129 - 1 - 3p was not related to FIGO stage,age,pathologic differentiation,lymph node metastasis,and serum level of CA125（P ﹥ 0. 05）. Conclusion：miR - 129 - 1 - 3p may participate the origination and progression of serous epithelial ovarian cancer as a tumor - supressor. It may represent potential indicator in the auxiliary of epithelial se-rous ovarian cancer and provide the theory basis to the biological targeted therapy of epithelial ovarian cancer.