李Ran基因的克隆与生物信息学分析

Cloning and Bioinformatic Analysis of Ran Genes from Prunus salicina Lindl.

本研究以芙蓉李为材料,采用同源克隆和RACE技术从芙蓉李叶片中分离得到2个李Ran基因PsRan1和PsRan3,长度分别为984 bp和952 bp。生物信息学分析结果表明,PsRan1和PsRan3均编码221个氨基酸。PsRan1和PsRan3与其他物种的Ran蛋白具有很高的相似性,均具有Ran蛋白的特征结构域,包括与GTP结合与水解有关的结构域、C-末端酸性结构域和影响因子结合结构域。NetPhos2.0Server预测结果表明二者具有10个相同的磷酸化位点。

Using Furong plum as experimental material, 2 PsRan genes, PsRan1 and PsRan3, were isolated through homologous cloning and RACE technology. PsRan1 and PsRan3 contained 984 bp and 952 bp in length respectively. Sequence analysis indicated that both PsRan1 and PsRan3 encode 221 amino acids. PsRan1 and PsRan3 showed high similarity with Ran proteins from other plants and contained characteristic domains of Ran proteins, including domains involved in GTP-binding and hydrolysis, as well as the acidic C-terminal domain and the effector-binding domain. Prediction of phosphorylation sites in these peptides using the NetPhos 2.0 server showed both of these PsRan proteins contained 10 identical phosphorylation sites.