混合型肝癌组织中OV_6与Snail蛋白的表达及临床意义

Expression and clinical significance of OV_6 and Snail in mixed hepatocellular cholangiocarcinoma

目的探讨混合型肝癌中OV6与Snail的表达及临床意义。方法回顾性分析本院1998年4月—2003年7月收治的经病理学诊断确诊为混合型肝癌且行手术切除的70例患者,采用免疫组织化学方法检测肿瘤组织中OV6和Snail蛋白的表达情况,分析其表达水平与临床病理因素及预后的关系。结果 OV6与Snail蛋白阳性表达率分别为42.8%(30/70)和47.1%(33/70);OV6和Snail蛋白的表达均与肿瘤数目、血AFP水平和微血管侵犯密切相关(P<0.05),且都影响患者术后生存(P<0.05)。Snail蛋白的表达还影响患者术后复发(P<0.05)。OV6和Snail在肿瘤中的表达呈正相关(r=0.270,P<0.05)。结论 OV6和Snail在混合型肝癌中的表达状态与肿瘤的发生发展密切相关。

Objective To explore the expression and significance of OV6 and Snail in mixed hepatocellular cholangiocarcinoma.Methods Clinical materials of 70 patients with mixed hepatocellular cholangiocarcinoma and surgical resection were analyzed retrospectively.Expressions of OV6 and Snail were detected by immunohistochemistry in tumor tissue,and the correlation between the expression levels and clinical pathological and prognosis were analyzed.Results The positive rates of OV6 and Snail were 42.8％ and 47.1％ respectively.The positive expression rates of OV6 and Snail were significantly related with tumor multiplicity,serum levels of oα-fetoprotein and microvascular invasion （P ＜ 0.05）,both affected overall survival.The protein expression of Snail also affected disease-free survival （P ＜ 0.0 5）.OV6 in tumor liver was associated with expression of Snail （r =0.2 7 0,P ＜0.05）.Conclusion Expressions of OV6 and Snail proteins are associated with clinicopathological development in mixed hepatocellular cholangiocarcinoma.