基质金属蛋白酶-9在子宫内膜癌组织中的表达水平及临床意义

Expression and clinical significance of matrix metalloproteinases-9 in tissues of endometrial carcinoma

目的探讨基质金属蛋白酶-9(MMP-9)在子宫内膜癌组织中的表达水平及临床意义。方法收集2009年2月—2012年3月在本院住院的30例子宫内膜癌组织石蜡标本设为实验组1,40例不典型增生内膜组织石蜡标本设为实验组2,40例正常子宫内膜组织石蜡标本设为对照组。应用免疫组织化学染色方法对各组标本进行MMP-9抗原染色,统计阳性染色细胞数量及染色程度。比较各组MMP-9表达情况;分析子宫内膜癌组织MMP-9表达与临床病理参数的关系;采用Cox比例风险模型分析子宫内膜癌预后生存因素。结果实验组1的低表达率明显低于实验组2和对照组,高表达率则明显高于实验组2和对照组(P<0.05);MMP-9蛋白表达情况与病理分级、淋巴结转移情况相关(P<0.05);病理分级、淋巴结转移状态是子宫内膜癌的预后影响因素(P<0.05)。结论 MMP-9在子宫内膜癌组织中特异性高表达,可作为诊断子宫内膜癌诊断及病理分级的参考指标之一,但其表达水平并非子宫内膜癌的独立预后影响因素。

Objective To investigate the expression and clinical significance of matrix metalloproteinases-9（ MMP-9） in tissues of endometrial carcinoma. Methods The paraffin samples of tissues in 30 patients with endometrial carcinoma admitted in our hospital from February 2009 to March2012 were selected as experimental group 1 while those in other 40 patients with atypical endometrial hyperplasia and 40 healthy people as experimental group 2 and control group,respectively. Immunohistochemical staining method was used for the MMP-9 staining so as to analyze the counts of cells with positive staining and the staining severity. MMP-9 expression levels were compared,the relationship between MMP-9 expression and clinical pathological parameters in endometrial carcinoma tissues were analyzed and Cox ratio risk model was applied to analyze the prognostic factors of endometrial carcinoma. Results Experimental group 1 was evidently lower in low-expression of MMP-9 than experimental group 2 and apparently higher in high-expression of MMP-9 than the other groups（ P〈 0. 05）. It was showed that pathological staging and lumph node metastasis were closely associated with MMP-9 protein expression（ P〈 0. 05）,which were also the risk factors impacting the prognosis of endometrial carcinoma（ P〈 0. 05）. Conclusion The specific high-expression of MMP-9 in endometrial carcinoma can be considered as one of the referent indexes for diagnosing endometrial carcinoma and its pathological staging,which,however,is not the independent risk factor influencing the prognosis of endometrial carcinoma.