参与不同牙本质粘接界面退变过程中基质金属蛋白酶种类的研究

Study of the types of matrix metalloproteinases involved in dentin bonding interface degradation

目的研究参与两种粘接剂形成的牙本质粘接界面退变过程中基质金属蛋白酶(MMPs)的种类。方法制备Single Bond 2和Clearfi l S3 Bond微剪切粘接试件,无菌人工唾液中分别浸泡0、6个月测试粘接强度,场发射扫描电镜(FE-SEM)观察界面断裂模式。制备4 mm×3 mm×1 mm牙本质片,分为对照组、Single Bond 2组和Clearfi l S3Bond组,无菌人工唾液浸泡0、6个月后,液相芯片技术测定各组MMP-1、-2、-3、-8、-9含量。结果老化6个月后,各组粘接强度显著下降。与对照组相比,老化过程中不同粘接剂组MMP-1、-3含量无明显变化,Single Bond 2组MMP-2、-8、-9含量显著降低,Clearfi l S3 Bond组MMP-8、-9含量显著降低。结论老化6个月后两种粘接试件均发生粘接界面退变,牙本质源MMP-2、-8、-9的含量显著降低,提示这些MMPs可能参与了牙本质粘接界面的退变。

Objective To study the types of matrix metalloproteinases （MMPs） involved in dentin bonding interface degradation. Methods Dentin slices were prepared and treated with two adhesive systems （Single Bond 2 or Clearfil S3 Bond）. The dentin surface was bonded with composite resin. All specimens were immersed in sterile artificial saliva for 0 or 6 months, and their micro-shear bond strength （μSBS） were measured. The fracture modes were observed through field emission scanning electron microscope （FE-SEM）. Dentin slices with 4 mm＆#215;3 mm＆#215;1 mm dimensions were prepared. The slices were divided into three groups according to the treatment modes （negative control, Single Bond 2, and Clearfil S3 Bond）. All specimens were stored in sterile artificial saliva for 0 or 6 months. The concentrations of MMP-1, -2, -3, -8, and -9 of each group were detected through fluorescent microsphere immunoassay. Results The μSBS of both adhesive systems significantly decreased after storage aging. Significant differences in failure modes within the four groups tested in this study were observed. Compared with the negative control, the concentrations of MMP-1 and MMP-3 in different adhesive groups showed no significant difference after storage aging. However, the concentrations of MMP-2, -8, and -9 in Single Bond 2 group and the concentrations of MMP-8 and -9 in Clearfil S3 Bond group significantly decreased after 6 months of storage aging. Conclusion Significant degradation occur in the dentin bonding interface of both adhesive groups under 6 months aging challenge. The concentrations of dentinal MMP-2, -8, and -9 significantly decrease after treatment with adhesives and aging, indicating that these MMPs have an important function in dentin bonding interface degradation.