绿原酸对颅脑损伤大鼠认知功能的保护作用

Protective effect of chlorogenic acid on cognitive function of rats with traumatic brain injury

目的探讨绿原酸对创伤性颅脑损伤大鼠认知功能的作用及可能机制。方法将24只SD大鼠随机均分为绿原酸组(A组)、模型对照组(B组)和假手术组(C组)。通过可控性皮层打击器建立大鼠颅脑损伤模型,用Morris水迷宫检测大鼠认知功能,ELISA法检测丙二醛(MDA)含量和脑源性神经营养因子(BDNF)表达。结果与C组相比,B组大鼠潜伏期延长,MDA水平升高,BDNF表达降低(P<0.05);而A组能有效逆转B组各观察指标的改变过程(P<0.05)。结论绿原酸可能是通过自身的抗氧化作用,降低MDA水平,提高BDNF表达,从而改善大鼠的认知功能。

Objective To investigate the effect and underlying mechanism of chlorogenic acid on the cognitive function of rats with traumatic brain injury. Methods Twenty-four SD rats were equally randomized into three groups of A （ injected with chlorogenic acid）, B （model control） and C （ sham operated）. Traumatic brain injury model was established by controlled cortical impactor, and the cognitive function was measured by Morris water maze. Malondialdehyde（MDA） content and brain- derived neurotrophic factor （BDNF） expression were detected by ELISA. Results Compared with group C, the latency stage of training was prolonged, MDA level was increased, while BNDF expression was decreased in group B （P〈0. 05 ）, which were significantly reversed in group A （P〈0. 05）. Conclusion Chlorogenic acid may decrease MDA level and increase BDNF expression via self antioxidation, which finally improves the cognitive function of rats with traumatic brain iniurv.