摘要
目的探讨β-连环蛋白(β-catenin)和富含亮氨酸的G蛋白偶联受体蛋白5(Lgr5)基因在先天性巨结肠(HD)中的表达。方法采用RT-PCR和Western blot检测30例HD患者正常肠段(有神经节细胞)和狭窄肠段(无神经节细胞)中β-catenin和Lgr5mRNA和蛋白表达,免疫组化检测β-catenin和Lgr5阳性表达情况。结果β-Catenin和Lgr5mRNA相对表达量在HD狭窄肠段中分别为17.66±1.28和15.01±1.29,低于正常肠段中的31.77±2.21和29.61±3.23(P<0.05)。β-Catenin和Lgr5相对蛋白含量在HD狭窄肠段中分别为19.51±1.57和17.35±0.87,低于正常肠段中的37.05±1.23和33.17±2.09(P<0.05)。β-Catenin和Lgr5在HD狭窄肠段中呈弱阳性或阴性表达。结论β-Catenin和Lgr5异常表达与HD发生发展相关。
Objective To investigate the expressions of β-catenin and Lgr5 genes in the bowels of Hirschsprung＇s disease（HD）. Methods The expressions of β-catenin and Lgr5 in normal bowels （with ganglion cells） and narrow bowels（without ganglion cells） were detected by RT-PCR and Western blot in 30 patients with HD. Results The relative levels of β-catenin and Lgr5 mRNA in narrow bowels were lower than those in normal bowels （ 17.66 ± 1.28 vs. 31.77 ± 2.21 and 15.01± 1.29 vs. 29.61±3.23）（P〈0. 05）. So did the relative contents of β-catenin and Lgr5 protein （19.51± 1.57 vs. 37.05±1.23 and 17.35~0.87 vs. 33.17±2.09） （P〈0.05）. Conclusion Abnormal expressions of β-catenin and Lgr5 are related to the occurrence and development of HD.
出处
《江苏医药》
CAS
北大核心
2014年第14期1675-1677,F0002,共4页
Jiangsu Medical Journal
