高效液相色谱-荧光检测法测定人血浆中洛美沙星的浓度

Measure of concentration of lomefloxacin in human plasma by high performance liquid chromatography-fluorescence assay

目的：建立用高效液相色谱-荧光检测法（HPLC-FLU）测定人血浆中洛美沙星的浓度。方法血浆样品经甲醇沉淀后,以甲醇-磷酸二氢钾缓冲液（pH：2.8,35：65, v/v）为流动相,流速为1.0 ml/min,色谱柱为BaiAn-C18（4.6×150 mm,5μm）,柱温为25℃,荧光检测波长λex=280 nm,λem=455 nm。结果血浆内源性杂质不干扰待测物测定,洛美沙星在0.10-10.04μg/ml有良好线性关系（r=0.9995）,定量下限为0.10μg/ml,日内、日间精密度（RSD）均小于10%,样品稳定性良好。结论该法灵敏、快速、简便,适合用于洛美沙星的药动学研究。

Objective To apply high performance liquid chromatography-fluorescence assay （HPLC-FLU） in measuring concentration of lomefloxacin in human plasma. Methods Plasma samples were precipitated by methanol. Methanol-monopotassium phosphate buffer solution （pH： 2.8, 35：65, v/v） was applied as mobile phase, and its flow velocity was 1.0 ml/min, chromatographic column was BaiAn-C18（4.6×150 mm, 5 μm）, column temperature was 25℃, and Fluorescence detection wavelength was λex=280nm and λem=455nm. Results The intrinsic impurity of plasma did not interfere with the measurement of determinand. Linearity of lomefloxacin was good at 0.10-10.04μg/ml （r=0.9995）, and the lower limit of quantitation was 0.10 μg/ml. The day-to-day and within-day relative standard deviation （RSD） were all less than 10%. Conclusion This measure method is fast, sensitive, and simple, which can be applied to the pharmacokinetic study of lomefloxacin.