β-咔啉类生物碱对人胃癌细胞SGC-7901增殖的影响

Effects of β-carboline of alkaloids on proliferation of SGC-7901 of gastric cancer cells in human

目的探讨β-咔啉类生物碱对人胃癌细胞SGC-7901增殖的影响。方法通过体外培养人胃癌细胞株SGC-7901,将含有不同浓度(5、10、20、40μg/mL)β-咔啉类生物碱的培养液与SGC-7901细胞共同培养24 h和48 h。采用MTT比色法计算细胞抑制率;在荧光显微镜下用Hoechst 33258细胞核染色法观察细胞形态学改变;流式细胞仪检测凋亡率及基因组DNA琼脂凝胶电泳检测凋亡梯状条带。结果β-咔啉类生物碱对SGC-7901细胞的损伤呈浓度依赖性;β-咔啉类生物碱分别作用24 h和48 h后半数抑制浓度(IC50)分别为17.79μg/mL和12.17μg/mL;荧光染色可观察到有细胞核固缩及核断裂的凋亡现象;流式细胞术显示:阴性对照组(0μg/mL)及β-咔啉类生物碱5、10、20、40μg/mL浓度组24、48 h凋亡率为别1.66%、11.27%、20.32%、30.66%、41.42%和3.84%、15.29%、23.34%、34.87%、49.54%,细胞凋亡率伴随给药浓度增加而增加;基因组DNA琼脂糖凝胶电泳检测到明显的凋亡梯状条带。结论β-咔啉类生物碱能诱导人胃癌SGC-7901细胞发生凋亡,抑制细胞增殖。

Objective To investigate the effects of β-Carboline of alkaloids on proliferation of SGC-7901 of human gastric cancer cells. Methods SGC-7901 cells were cultured in vitro initially.After SGC-7901 cells were incubated with β-Carboline alkaloids at different concentrations of 5,10,20,40 μg /mL for 24,48 hours,the inhibited proliferation rate of SGC-7901 cells were examined by Methtl Thiazolyl Tetrazolium（MTT） assay. Morphological changes of SGC-7901 cells were observed by Hoechst 33258 under fluorescence microscope. Flow cytometry was used to detect cell apoptosis after SGC-7901 cells were incubated with β-Carboline alkaloids for 24 hours. Cell gemonic DNA was detected by agarose electrophoresis. Results β-Carboline of alkaloids induced damage of SGC-7901 cell in a concentration dependent manner. The IC 5 0 of β-Carboline alkaloids at 2 4,4 8 hours were17. 79 μg /mL and 12. 17 μg /mL respectively. Apoptotic cells were observed and flow cytonetry analysis in dicated that the apoptosis rate of cells treated with different concentrations of β-Carboline alkaloids（0,5,10,20,40 μg /mL） for 24 and 48 hours were 1. 66%,11. 27%,20. 32%,30. 66%,41. 42%; 3. 84%,15. 29%,23. 34%,34. 87%,49. 54%,respectively. Increasing in apoptosis rate of SGC-7901 cells was associated with drug concentration. Typical DNA Ladder was detected in DNA agarose electrophoresis. Conclusion β-Carboline alkaloids can inhibit the proliferation and induce the apoptosis of SGC-7901 cells.