鸡新城疫、传染性支气管炎二联活疫苗耐热保护剂筛选和优化

Selection and optimization of thermostable agent for Newcastle Disease and Infectious Bronchitis bivalent vaccine

应用冻干制品加速热稳定试验，测试7组冻干保护剂配方对鸡新城疫、传染性支气管炎二联活疫苗（LaSota株＋H120株）的耐热保护效果。试验筛选出用于鸡新城疫、传染性支气管炎二联活疫苗的耐热保护剂，使用该保护剂的疫苗表现为37℃保存10d，病毒含量下降0．4个滴度，常规疫苗下降2～2．4个滴度；25℃保存30d，病毒含量下降0．4～0．6个滴度，常规疫苗下降1．8～2．6个滴度。证明此保护剂具有良好的耐热效果。

In this paper, we tested protective effects of 7 groups of the lyoprotectant recipe for Newcastle Disease and Infectious Bronchitis Vaccine, Live Vaccine （La Sota strain ＋ H120 strain） were used for cross reference by an accelerated stability test. The best lyoprotectant recipe of Newcastle-Bronchitis Vaccine had been screened in this experiment, and the results indicated that when exposure this lyoproteetant vaccine at 37 ℃ for 10 days, or exposure at 25℃ for 30 days, the vaccine virus titers declined by 0.4 unit or 0.4-0.6 unit respectively, while other lyoprotectant vaccines virus titers decreased by 2-2.4 unit or 1.8-2.6 unit respectively. In conclusion, the new lyoprotectant showed effective protection for Newcastle-Bronchitis Vaccine.