鹿血多肽的制备工艺及抗氧化能力研究

Study on the preparation process and the antioxidant capacity of deer blood polypeptide

以新鲜鹿血为原料,利用胰蛋白酶水解制备鹿血多肽,以水解度为指标,在单因素和正交实验的基础上,确定酶解的最佳工艺条件,即加酶量为6%,pH为9,反应温度为37℃,反应时间为4h,水解度为46.72%±0.11%。并进一步对鹿血多肽进行抗氧化能力测定,在高浓度时多肽的羟自由基清除能力和DPPH自由基清除能力较高。

The extraction of deer blood polypeptide was studied using trypsin hydrolysis with resh deer blood as aw material.This article took the degree of hydrolysis as index.The optimum conditions for enzymatic hydrolysis was determined on the basis of single factor and orthogonal experiment.The optimum conditions were the amount of enzyme 6%, prig, the hydrolysis temperature 37℃, reaction time 4h, which the degree of hydrolysis reached 46.72% ± 0.11 % under this condition.And determination of antioxidant capacity of the peptides were performed.The high concentration of deer blood polypeptide had strong hydroxyl radicals scavenging ability and DPPH radical scavenging ability.