摘要
建立了脂溶性天然色素中特丁基对苯二酚(TBHQ)的检测方法。样品经凝胶渗透色谱(GPC)技术去除色素和脂类,高效液相色谱荧光法进行定性、定量分析。加标水平为0.5—1.5mg/kg时,TBHQ的回收率为85.50%~103.33%。相对标准偏差为1.06%-7.04%。方法检出限0.1mg/kg。本方法具有净化效果好,准确灵敏,回收率高等优点,适用于脂溶性天然色素中特丁基对苯二酚的检测。
A novel method was developed for the determination of tert-butylhydroquinone (TBHQ)in fat-soluble pigments using gel permeation chromatography (GPC) and high performance liquid chromatography with fiuorometric detector.The average recoveries of TBHQ were 85.50% ~ 103.33%, spiked at the level of 0.5-1.5mg/kg. The relative standard deviations were in the range of 1.06%-7.04%, and the procedure allows the detection of 0.1mg/kg.The method was rapid, precise and high efficient. It can be extended to the analysis for TBHQ in fatsoluble pigments.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第17期308-311,350,共5页
Science and Technology of Food Industry
基金
国家国际科技合作专项(S2013ZRO319)
关键词
凝胶渗透色谱
高效液相色谱荧光法
特丁基对苯二酚
脂溶性天然色素
gel permeation chromatography
high performance liquid chromatography with fluorometric detector
tert- butylhydroquinone
fat-soluble pigments