乌贼墨多糖对环磷酰胺致小鼠卵巢损伤的保护作用

Protective effects of squid ink polysaccharides against cyclophosphamide-induced ovarian damage in mice

研究乌贼墨多糖对注射环磷酰胺所致小鼠卵巢功能损伤的保护作用。本研究用雌性昆明小鼠作为模型动物,以乌贼墨多糖作为卵巢保护剂进行灌胃,腹腔注射环磷酰胺造模,检测实验小鼠的体重、卵巢指数、血清中性激素水平以及卵巢的抗氧化指标,对卵巢进行组织病理学检测并统计各级生长卵泡数量。结果显示,乌贼墨多糖能够明显缓解环磷酰胺对卵巢的毒性损伤,降低血清中FSH水平(p<0.05)和LH水平(p<0.01),升高血清中E2水平(p<0.01),提高卵巢组织中SOD活力(p<0.05)、GSH-Px活力(p<0.01)以及GSH含量(p<0.01),降低卵巢中MDA含量(p<0.01),显著增加原始卵泡数、初级卵泡数、成熟卵泡数以及卵泡总数(p<0.01)。由此表明,乌贼墨多糖可明显抑制环磷酰胺造成的卵巢损伤,保护并修复卵巢的组织结构,为其开发成临床化疗的辅助药物提供理论依据。

To investigate protective effects of squid ink polysaccharide（SIP）on ovary injured by cyclophosphamide （CP）.ln this paper,female Kunming mice were used as model animals,which were administered orally with SIP as ovarian protection agent gavage to against ovarian failure caused by intraperitoneal injection of CP.The mouse body weight and ovarian index,levels of FSH, LH, E2 and T in serum, activities of SOD, GSH-Px and contents of GSH,MDA in ovary, histopathology,numbers of follicles were detected.Results showed that the negative roles of CP on ovary were significantly intervened by SIP,which recovered levels of FSH（ p 〈 0.05）, LH and E2 （p 〈 0.01 ）in serum, significantly increased activities of SOD（ p 〈 0.05） and GSH-Px（ p 〈 0.01 ） as well as GSH content（ p 〈 0.01 ） in ovary,and decreased MDA level （p 〈 0.01 ）. Meanwhile,there were obvious increased in numbers of primordial follicles, primary follicles, mature follicle and the total number of follicles, and ovarian structure was made a distinct improvement.it was suggested that SIP could weaken ovarian damage caused by CP,which implyed a potential medicinal value of the marine bioactive polysaccharide.