应用微电极芯片技术评价抗心房颤动药物对兔右心房快速起搏模型的影响

Evaluation of Anti-atrial Fibrillation Drug With Multi Ion Channel Targets by Micro-electrode Chip Technology in Experimental Rabbit Model

目的:应用微电极芯片技术筛选和评价抗心房颤动(房颤)药物对兔右心房快速起搏模型的影响。方法:兔32只随机分为4组:钾离子通道阻断剂Tetraethylammonium组(TEA组,n=8),钾离子通道阻断剂氯化钡组(BaCl2组,n=8),钾离子通道阻断剂氯化镉组(CdCl2组,n=8),胺碘酮组(n=8)。经颈内静脉将电极置入右心房,以600次/分行心房快速起搏(RAP),给予TEA、BaCl2、CdCl2和胺碘酮观察抗房颤药物作用的离子通道对电生理指标场电位时程(fAPD)的影响。结果:24小时快速起搏后,20 mmol/L TEA使场电位时程由(176.67±8.66)ms延长到(196.11±10.76)ms(P=0.012)。1×10-4mol/L BaCl2使场电位时程由(182.22±12.87)ms延长到(191.11±13.09)ms(P=0.039)。CdCl2使场电位时程由(178.33±7.85)ms延长到(206.67±9.70)ms(P=0.0015)。2×10-6 mmol/L胺碘酮灌流心脏组织,场电位时程由(167.38±13.67)ms延长到(185±15.14)ms(P=0.002),差异有统计学意义。结论:快速起搏兔右心房致房颤模型是一种简单可行的抗房颤药物筛选模型,结合微电极芯片技术有可能用于抗房颤药物开发的早期快速筛选。

Objective：To evaluate and screen the anti-atrial ifbrillation drug with multiion channel targets by micro-electrode chip technology in a rapid atrial pacing （RAP） rabbit model. Methods：A total of 32 rabbits were randomly divided into 4 groups, n=8 in each group. Potassium channel blocker （TEA） group, Potassium channel blocker （BaCl2） group, Potassium channel blocker （CdCl2） group and Amiodarone group. The electrode was inserted into right atrium via internal jugular vein with rapid right atrial pacing （600 beat/min） and the effect of each anti-atrial ifbrillation drug on ifeld action potential （fAPD） were measured in different groups. Results：With 24 hour RAP, the fAPD was prolonged from （176.67 ± 8.66） ms to （196.11 ± 10.76） ms, P=0.012 in TEA group;from （182.22 ± 12.87） ms to （191.11 ± 13.09） ms, P=0.039 in BaCl2 group;from （178.33±7.85） ms to （206.67 ± 9.70） ms, P=0.0015 in CdCl2 group;from （167.38 ± 13.67） ms to （185 ± 15.14） ms, P=0.002 in Amiodarone group. Conclusion： RAP induced atrial fibrillation in experimental rabbit model is a simple and feasible method for screening the anti-atrial fibrillation drugs, combining with micro-electrode chip technology, it might be used for developing the new product.