流式细胞技术(FCM)在人乳腺癌耐药细胞系耐药逆转研究中的应用

Application of flow cytometry in reversal of multi-Drug resistance in breast cancer cell line

目的:人乳腺癌耐药细胞系(MCF-7/ADR)经不同逆转方法作用后,对耐药逆转结果进行检测,寻找最佳逆转方法。方法:用流式细胞仪,分别对MCF-7及经不同逆转剂作用后MCF-7/ADR进行检测。结果:经流式细胞仪检测,P-170表达:MCF-7为11.4％,MCF-7/ADR为99.2％,不同逆转剂作用细胞后各实验组P-170表达均大幅度下降。经PI染色后,不同逆转剂逆转MCD-7/ADR的细胞周期无明显变化。结论:异博定结合干扰素及加温的方法,逆转效果最佳。

Objective: Human breast cancer cell line with drug resistance (MCF-7/ADR) was treated by kinds of reversal methods. To detect the result and investigate the best reversal methods. Methods: The P-glycoprotein ( P-gp) , a product of the multi-drug resistance gene was detected by flow cytometry. Results: The positive rate of P-glycoprotein on breast cancer cell line MCF-7 and MCF-7/ADR was 11.4% and 99.2% , respectively.The expression of P-glycoprotein of MCF-7/ADR was significant descent in the experiment groups, but the distribution of cell cycle did not change through detecting the result of PI stain ing. Conclusion: The reversal action of Interferon-α combined with Verapamil and hyperthermia was the best method in breast cancer cell line MCF-7/ADR.