HPLC法测定儿咳颗粒中甘草酸的含量

Determination of Glycyrrhizic Acid in Erke Granules by High Performance Liquid Chromatography

目的：应用HPLC法测定儿咳颗粒中甘草酸的含量。方法：采用高效液相色谱法,合理选择色谱柱为Kromasil C18（5μm,200 mm×4.6 mm）,流动相为乙腈-0.2%醋酸（63∶37）,流速为0.8 mL/min,检测波长为250 nm。结果：甘草酸在0.0513~0.3076 mg/mL范围内呈线性关系,平均回收率为99.71%,RSD=0.65%（n=6）。结论：HPLC法测定儿咳颗粒中甘草酸方法可靠、准确、灵敏、重现性好,可以对儿咳颗粒中甘草酸的含量进行更好的控制。

Objective：To determine the content of glyeyrrhizic acid by high performance liquid ehromatography（HPLC） in Erke granules. Methods：Using HPLC,the reasonable selection of chromatographic column was Kromasil C18 column（5 μm,200mm×4.6 mm）,acetonitrile-0.2% acetic acid（63：37） as mobile phase. The flow rate was 0.8 mL/min,and the detective wavelength was 250 nm. Results：The calibration curves was linear between 0.0513--0.3076 mg/ml,and the average recovery was 99.71%,RSD=0.65%（n=6） for glycyrrhizic acid. Conclusion：HPLC method determination of glycyrrhizic acid in Erke granules is reliable,accurate,sensitive, repeatable,and the content control of glycyrrhizic acid can be better for Erke granules in the particles.