MDM2和SPIN1mRNA在喉癌组织中的表达及其相关性研究

Study on expressions of MDM2 and SPIN1mRNA in cancer of larynx

目的:研究喉癌组织中MDM2和SPIN1mRNA的表达,探讨其相关性及在肿瘤发生发展中的作用。方法:应用real-time PCR对32例喉癌及癌旁组织标本的MDM2、SPIN1在mRNA水平进行检测。结果:MDM2在喉癌组织中的表达明显高于癌旁正常组织,差异具有显著性(P<0.01);SPIN1在喉癌组织和癌旁正常组织中表达无显著性差异(P>0.05)。MDM2在喉癌中的表达与肿瘤的病理分化呈负相关(P<0.01)。SPIN1的表达和肿瘤分化无关(P>0.05)。喉癌组织中MDM2和SPIN1表达无显著相关性(P>0.05)。结论:MDM2基因在喉癌的发生发展中可能发挥重要作用,而SPIN1基因在喉癌的发生发展过程中可能不发挥主要作用,两者之间的相互作用机制有待进一步研究。

Objective：To observe the expressions of MDM 2 and SPIN1 mRNA in tissue of cancer of larynx and investigate their correlations and explore their roles in tumor genesis .Method ：The expressions of MDM2 and SPIN1mRNA in 32 cancer of larynx tissues and adjacent tissues detected by RT-PCR .Result ：There were significant differences in the expressions of MDM2 of 32 samples ,MDM2 mRNA presented higher level expression in cancer of larynx tissues （P〈 0 .01） ,while SPIN1mRNA expressions of two tissues show no significant difference . MDM2 mRNA expression increased along with the increased tumor differentiation degree of cancer of larynx （P〈0 . 01） .There were no correlation between the expressions of MDM 2 and SPIN1 in cancer of larynx .Conclusion ：MDM2 may play an important role in tumor genesis of cancer of larynx ,while SPIN1 may play an unimportant role in tumor genesis of cancer of larynx .Their relationship will be researched in further study .