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酸土脂环酸芽孢杆菌DnaK基因克隆及生物信息学分析 被引量：1

Cloning and Bioinformatics Analysis of DnaK Gene in Alicyclobacillus acidoterrestris
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摘要 利用同源克隆和基因组步移技术克隆得到酸土脂环酸芽孢杆菌(DSM 3922T)DnaK基因全序列,其ORF全长1 854 bp,编码617个氨基酸,推测其氨基酸序列与来源于A.acidocaldarius LAA1的分子伴侣蛋白DnaK同源性最高,相似性达86%。利用同源建模对DnaK的二级结构和超二级结构的预测结果显示,该伴侣蛋白主要由α-螺旋和β-折叠组成,基本不含无规则卷曲,具有耐热蛋白质的特征。 This paper had obtained complete sequence of DnaK gene from Alicyclobacillus acidoterrestris（DSM3922T） by applying homologous cloning and genome-walking. The DnaK gene contains a complete ORF of 1 854 bp, encoding 617 aa, which deduced amino acid sequence shared the highest homology with DnaK from A. acidocaldarius LAA1（86%）. Homology modeling had used to predict the secondary and super-secondary structure. The results showed that A. acidoterrestris DnaK is mainly composed of alpha spiral and beta folding. It has little random coil and characteristics of thermostable protein.
作者 刘明鹏 焦凌霞 李刚 梁新红 孙俊良
机构地区 河南科技学院食品学院
出处 《中国食品学报》 EI CAS CSCD 北大核心 2014年第7期199-206,共8页 Journal of Chinese Institute Of Food Science and Technology
基金 河南省高等学校青年骨干教师项目(2011GGJS-131)
关键词 酸土脂环酸芽孢杆菌 DnaK基因 克隆 生物信息学分析 Alicyclobacillus acidoterrestris DnaK gene cloning bioinformatics analysis
分类号 Q78 [生物学—分子生物学] TS201.3 [轻工技术与工程—食品科学]
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参考文献18

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二级参考文献3

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同被引文献28

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中国食品学报
2014年 第7期

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