虎耳草颗粒的质量控制方法

A Quality Control Method for the Saxifraga Granules

目的 建立虎耳草颗粒的质量控制方法。方法 色谱柱为hypersil ODS（250 mm×4.0 mm、5μm）,流动相为甲醇-水（20∶80）,流速为1.0 mL/min,检测波长275 nm,柱温30℃。结果 岩白菜素的进样量在0.0202-0.202μg之间与峰面积呈良好线性关系（R2=0.9998）,平均回收率为100.33%,RSD=1.92%。结论 本方法灵敏、准确、重现性好,可用于虎耳草颗粒的质量控制。

Objective To establish a quality control method of Saxifraga granules. Methods The analysis was performed on phenomenex hydro C18 column （250 mm×4.6 mm, 4 μm）, the mobile phase was MeOH-H20 （20 ： 80） with a flow rate at 1.0 mL/min, and detectional wavelength at 275 nm under column temperature 30 ℃. Results There was a good linear relationship when the sample size of bergenin was at a range of 0.0202-0.202μg（R^2=0.9998）, the recovery was 100.33%, with RSD at 1.92%. Conclusion This method was sensitive, exact, and reproducible to be used for the quality control of the Saxifraga granules.