小球藻吸收固定CO_2的研究

Absorption and fixation of carbon dioxide by Chlorella sp.

为研究小球藻吸收转化CO2的适宜条件,提高小球藻对CO2的利用率及生物转化量,在同一光照条件下,建立了光生物反应器培养小球藻的光衰减模型,研究通气速率、入口CO2的体积分数、初始pH、藻液接种质量分数等因素对小球藻固碳效果的影响。结果表明:光衰减系数与藻细胞浓度呈线性关系,小球藻光衰减模型为I=I0exp[-(0.104 9+0.336 5OD685)L],当通气速率为200 mL/min,入口CO2体积分数为10%,初始pH为9.0,初始藻液质量分数为0.1时,小球藻生长速率、生物量产率、固碳率及叶绿素含量均较高。结果对微藻固碳效率及机制研究有重要的参考价值。

To obtain the optimal conditions for absorption and transformation of CO2 using Chlorella sp.,a light attenuation model in the tank photobioreactor is established.The effects of aeration rate,volume fraction of inlet CO2,initial pH and mass fraction of initial microalgae on fixation of CO2 in a certain light are studied.The results show that light attenuation coefficient and microalgae cell concentration are linear relation and the light attenuation model of Chlorella sp.is I =I0 exp [-（0.104 9 ＋ 0.336 5OD685）L].Cell growth rate,biomass productivity,CO2 fixation rate and chlorophyll content are relatively high under the following conditions：200 mL/min of aeration rate,10％ volume fraction of inlet CO2,initial pH 9.0,and 0.1％ mass fraction of initial microalgae.These results may have an importantrole in further investigation of CO2 fixation efficiency and mechanism using microalgae.