醋酸纤维素手性固膜对R,S-扁桃酸的拆分研究

Enantiomeric separation of(R,S)- mandelic acid using cellulose acetate membrane

醋酸纤维素已经被广泛用于膜分离技术及用作高效液相色谱手性固定相,显示了它具有优良的成膜性能和手性识别能力。因此以醋酸纤维素为膜材料,制备醋酸纤维素手性固膜,使用R,S-扁桃酸作为原料液检测膜的通量及其手性拆分能力,并使用扫描电子显微镜对膜表面及截面的形态结构进行了表征。研究显示:当CA浓度为30%,DMF浓度为15%,铸膜液挥发5min,操作压力为0.2 MPa,样品浓度为0.5 mg/mL时,膜具有良好的拆分效果,R,S-扁桃酸对映体的分离因子可以达到3.9,说明膜分离技术是进行大规模手性拆分非常有潜力的方法之一,具有良好的工业应用前景。

Since cellulose acetate has been widely used for membrane separations and chiral stationary phase in HPLC, It showed favorable film-forming performance and enantiomeric recognition potency, an enantioselective membrane was prepared using cellulose acetate as the membrane material. The flux and permselective properties of a membrane were studied using mandelic acid racemate as the feed solution. The top surface and cross-section morphology of the resulting membrane were examined using scanning electron microscopy. An optical resolution of at 3.9 enantiomeric separation factor was achieved when the enantioselective membrane was prepared with 30wt.% cellulose acetate, 15wt.% N, N-dimethylformamide in the casting solution of acetone, the evaporation time of 5 minutes and operating pressure of 0.2 MPa. This work indicates that the enantioselective cellulose acetate membrane could soon become very attractive for industrial uses.