药用植物茅苍术工厂化育苗关键技术研究

Research on the Key Techniques in Factory Breeding of Medicinal Plants of Atractylodes Lancea(Thunb.) DC.

建立茅苍术高频快繁和试管苗移栽技术体系,为种苗的大规模工厂化生产提供技术依据。以野生茅苍术种子为试验材料,用0.1%的HgCl2消毒后建立无菌系,采用均匀设计法对茅苍术的增殖及生根培养基进行优化,并对试管苗移栽基质进行筛选。结果表明:采用0.1%的HgCl2灭菌4 min,种子萌动率达87%;茅苍术增殖最佳培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,30 d的平均增殖系数达10.00±0.71;生根最佳培养基为1/2 MS+NAA 1.0 mg/L,平均生根条数达7.60±0.40;试管苗在蛭石基质中成活率达到91%。该研究建立的茅苍术种苗大规模工厂化生产技术体系,为茅苍术人工栽培所需大量种苗的工业化生产奠定了基础。

To establish technical system of rapid propagation and tube seedlings transplanting of Atractylodes lancea DC. and to provide the technical basis for establishing its industrialized breeding sterile system, the wild seeds of Atractylodes lancea DC. were disinfected with 0.1% HgC12 and the optimization system of its proliferation and rooting medium was studied through the uniform design. The results showed that seed stirring rate was 87% after pretreatment with 0.1% HgCl2 for 4 min. The best culture medium suitable for the proliferation of Atractylodes lancea DC. was MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.2 mg/L, and the average multiplication factor was 10.00 ＋ 0.71 every 30 days ;The optimum medium for rooting was 1/2MS ＋ NAA 1.0 rag/L, and the average root number was 7.60 ＋0.40;The highest survival rate of the tube seedlings was 91% in vermiculite. This research established the technical system of seedlings for large-scale factory production of Atractylodes lancea DC, which laid a solid foundation for a large number of seedlings neoded in the artifieal cultivaton in industrial production of Atractylodes lancea DC.