超富集植物商陆多倍体诱导研究

Study on Polyploid Induction of Hyperaccumulator Plant—Phytolacca acinosa Roxb.

以商陆为试材,对商陆幼苗进行秋水仙素诱导多倍体试验。结果表明:0.2%秋水仙素诱变48h效果最好,与对照相比,变异株茎粗壮,叶宽、叶厚较对照分别增加40.15%和33.25%;气孔纵横径较对照分别增加30.25%和60.82%,气孔密度增加150%;压片法对变异植株和对照植株的根尖有丝分裂分别进行染色体鉴定,证明变异株为多倍体。

Taking Phytolacca acinosa Roxb. as material, Phytolacca acinosa Roxb. seedlings were treated with different concentrations of colchicines solution,polyploid induction test was conducted. The results showed that the best treatment was using 0. 2% colchicines solution and 48 hours. Compared with the control,the stems of variant plants became more dwarf and stronger,leaf width was increased by 40. 15% and leaf thickness was increased by 33. 25%. Length and width of stomata was increased by 30. 25 % and 60.82 % respectively,the density of stomata was increased by 150% ;the variant plants were polyploid by tabletting method of mutant plants and control of the plant root mitotic chromosome identification respectively.