蓝莓叶穿孔病菌鉴定及其生物学特性研究

Study on Identification and Biological Characteristics of Caused Agent of Leaf Perforation on Blueberry

以蓝莓产区发现的一种新病害蓝莓叶穿孔病样为供试病菌,采用柯赫氏证病法则、形态学测定、ITS和EF-1α序列比对鉴定和常规病原菌生物学特性研究技术,研究了该病的致病菌,致病菌的分类地位、致病菌在不同培养条件下菌丝生长和孢子萌发的特性。结果表明:引起蓝莓叶穿孔病的致病菌为Alternaria alternata,该菌在供试条件下菌落生长以PDA为最优培养基,30℃为最佳生长温度,最佳pH 5,最佳氮源为甘氨酸,最佳碳源为葡萄糖;孢子萌发最佳条件为30℃,pH 5,2%甘氨酸,2%葡萄糖。病原菌菌丝致死温度为56℃、10min。

Thking sample of leaf perforation identified in blueberry production areas as test material, based on Koch＇s postulates, morphological characters, rDNA-ITS and EF-1α sequences blust and biological characters method, the pathogenic bacteria, classification status of pathogenic bacteria, pathogen mycelia growth and spore germination under different culture conditions were studied. The results showed that the pathogen was identified as Alternaria alternata, the mycelia grew best on PDA media, under 30℃, pH 5, dextrose and glycine were the best carbon and nitrogen source respectively. The spore germination condition was 30℃ ,pH 5,2% glycine and 2% dextrose suspension, the mycelia died under 56℃ for 10 min.