黄芩悬浮细胞培养过程中黄芩苷含量与苯丙氨酸解氨酶活性分析

Analysis of Content of Baicalin and Activity of Phenylalanine Ammonia Lyase from Cell Suspension Cultures of Scutellaria baicalensis

以黄芩悬浮细胞为试材,研究了悬浮培养过程中黄芩苷含量与苯丙氨酸解氨酶活性变化,采用均匀设计法分析了不同激素配比对黄芩生物量积累的影响。结果表明:黄芩细胞悬浮系生长呈较典型的"S形"曲线,黄芩苷变化与生物量积累基本一致;在黄芩悬浮培养周期中,苯丙氨酸解氨酶的活性强弱与黄芩苷的积累有一定相关性,培养第12天时活性最强。经均匀设计实验法得到最适宜黄芩悬浮培养生物量积累的激素配比为MS培养基附加6-BA 0.75mg/L,NAA0.5mg/L,KT 1.5mg/L。

Using cell suspension cultures of Scutellaria baicalensis as materials, the change of baicalin contents and activities of phenylalanine ammonialyase （PAL） from cell suspension cultures of Scutellaria baicalensis were analyzed in this paper. The effects of plant hormone proportions on biomass accumulation of the cell suspension cultures were studied by uniform design. The results showed that the growth curves of the cell suspension cultures exhibited ＇S＇ curve, and biomass accumulation of the cell suspension cultures matched with change of baicalin contents. There was correlation between PAIr activities and change of baicalin contents. The most suitable hormone ratio to biomass accumulation of the suspension cultures was MS medium added with 6-BA 0. 75 mg/L, NAA 0. 5 mg/L, KT 1.5 mg/L by uniform design method.