甘草皂苷对小鼠急性胰腺炎疗效及机制研究

The effect and mechanism of Saponins of Radix Glycyrrhiza in mice with acute pancreatitis

目的探讨了甘草皂苷对小鼠急性胰腺炎的治疗效果和机制。方法 60只BalB/c小鼠随机分为对照组、模型组、低剂量甘草皂苷组和高剂量甘草皂苷组。采用腹腔注射100μg/kg的雨蛙肽建立小鼠急性胰腺炎模型。低剂量甘草皂苷组和高剂量甘草皂苷组分别于建模前3 h腹腔注射甘草皂苷15 mg/kg和45 mg/kg。分别于建模后8、16和24 h处死小鼠。采用全自动生化分析仪检测小鼠血清淀粉酶(AMY),采用ELISA检测血清中TNF-α和IL-6的水平,采用HE染色观察小鼠胰腺组织病理学变化,采用Western blot检测胰腺组织中HMGB1蛋白的表达情况。结果与对照组比较,模型组不同时间点血清AMY水平、TNF-α和IL-6炎症因子水平、胰腺组织病理学损伤及胰腺组织中HMGB1蛋白表达水平随着时间延长而逐渐增加。与模型组比较,低剂量组和高剂量组不同时间点血清AMY水平及TNF-α和IL-6炎症因子水平明显下降,胰腺组织病理学损伤明显减轻,而胰腺组织中HMGB1蛋白表达水平明显下降(P<0.05)。与低剂量组比较,高剂量组上述指标和症状改善更为显著(P<0.05)。结论甘草皂苷可显著改善急性胰腺炎小鼠的炎症水平,减轻胰腺组织损伤,可能通过下调HMGB1蛋白来实现。

【Objective】To explore the effect and mechanism of Saponins of Radix Glycyrrhiza in mice with acute pancreatitis. 【Methods】Sixty Balb/c mice were randomized into control group, model group, low dose group and high dose group. Acute pancreatitis mice wereinduced by intraperitoneal injection of 100 μg/kg caerulein. Mice in low dose group and high dose group were treated with 15 mg/kg and 45 mg/kg Saponins of Radix Glycyrrhiza at 3 h before model construction. Mice in four group were sacrificed at 8, 16 and 24 hours. Serum level of amylase（AMY）was determined by automatic biochemistry analyzer. TNF-α and IL-6 were measured by ELISA. Pancreatic histopathological changes were observed by HE staining. Expressions of HMGB1 in pancreas were detected by Western blotting. 【Results】 Compared with control group, Serum AMY,TNF-α and IL-6 level, pancreatic histopathological changes and expressions of hmgb1 in pancreas in model group with the prolongation of time were significantly enhanced（P 0.05）. Compared with model group, serum AMY,TNF-α and IL-6 level and expressions of hmgb1 in pancreas in low dose group and high dose group significantlywere reduced, and pancreatic histopathological changes in low dose group and high dose groupwere significantly improved（P 0.05）. Compared with low dose group, the index above of all in high dose group were more significantly improved（P 0.05）.【Conclusion】Saponins of Radix Glycyrrhiza can improve inflammation level and alleviate histopathological changes bydown-regulating HMGB1 protein in mice with acute pancreatitis.