不同中药提取物对胰岛素抵抗L6肌细胞葡萄糖摄取率的影响

Effect of herbal extracts on insulin-resistant L6 cell and potential mechanisms

目的研究不同中药提取物对构建的L6细胞胰岛素抵抗性的影响。方法构建L6胰岛素抵抗细胞。将大鼠L6肌细胞分为9组:正常L6细胞组(NC)、胰岛素抵抗组(IR)和7组不同中药处理实验组(IRDrug Treated)。随后测定各组细胞葡萄糖摄取量,评估各中草药对胰岛素抵抗细胞葡萄糖代谢影响。结果随着时间的延长,IR组细胞葡萄糖摄取率比NC组细胞葡萄糖摄取率显著下降6.4%(P<0.05)。IR组与各实验组对比结果显示:细胞培养6 h时,实验组细胞中只有小檗碱组细胞葡萄糖摄取率比IR组显著上升(P<0.05),其他组细胞葡萄糖摄取率与IR组之间并无明显差异(P>0.05)。细胞培养12 h,中药提取物处理组中小檗碱、葛根素、黄芪多糖、麦冬多糖和大黄素组细胞葡萄糖摄取率较IR组均显著上升(P<0.05),且这种显著性随着培养时间的延长逐步增强;而人参皂苷组细胞和银杏叶提取物组细胞葡萄糖摄取率虽略有上升,但差异并不显著(P>0.05)。结论小檗碱、葛根素、黄芪多糖、麦冬多糖和大黄素均可通过增强IRS-1酪氨酸磷酸化来增强胰岛素抵抗L6细胞葡萄糖摄取能力。

[ Objective ] Construction of insulin resistance in mice L6 muscle cells to study the effects of different herbal extracts of L6 cells insulin resistance. [ Methods ] Build L6 cells insulin resistance. The rats were divided into nine groups L6 muscle cells： normal L6 cell group （NC）, insulin resistance group （IR） and 7 different sets of experimental medicine treatment group （IR-Drug Treated）. Then measure cells glucose uptake rate in each group, assess the effects of herbal resistant cells metabolize glucose to insulin. [ Results ] Cell culture 6h, IR group were significantly decreased glucose uptake rate 6.4%（P 〈0.05） than NC cells , and with time, which gradually increased significantly （P 〈0.05）. IR group and the experimental group comparison showed that： when the cell culture 6h, the ex- perimental group were only berberine ceils glucose uptake increased significantly compared with IR group （P 〈0.05）, among other groups cell glucose uptake and IR group no significant difference（P 〉0.05）. Cell culture 12h, herbal extracts treated berberine, puerarin, APS, polysaccharides and emodin group cell glucose uptake rate than IR group were increased significantly （P 〈0.05）, and this was significant as the culture extension of time gradually increased;while ginsenosides cells and Ginkgo biloba extract cells, although a slight increase in the rate of glucose uptake, but the difference was not significant （P 〉0.05）. [ Conclusions ] Berberine, puerarin, APS, polysaccharides and emodin insulin resistance can be enhanced glucose uptake in L6 ceils by enhancing the ability of IRS-1 tyrosine phosphorylation.