西沙海绵Agelas aff.nemoechinata化学成分研究

Studies on chemical constituents of Xisha sponge Agelas aff.nemoechinata

目的 研究西沙海绵Agelas aff.nemoechinata的化学成分。方法 利用Sephadex LH-20凝胶色谱、硅胶色谱、ODS色谱、HPLC等手段对化学成分进行了分离纯化;通过理化性质,波谱分析方法结合文献对照,鉴定化合物的结构。结果 从南海海绵甲醇溶剂提取物中,分离鉴定了8个单体化合物：尿嘧啶（1）、胸腺嘧啶（2）、1 H-2-吡啶酮（3）、胸腺嘧啶-2’-脱氧核苷（4）、2-甲酰胺吡咯（5）、3-醛基吲哚（6）、6-羟基-4,4,7a-三甲基-5,6,7,7a-四氢苯并呋喃-2（2 H）酮（7）、5-氨基戊酸（8）。结论 化合物1~8均为首次从该种海绵中分离得到。化合物7在50μg·mL-1浓度水平,对K562白血病细胞株、P388白血病细胞株和HeLa肿瘤细胞株的抑制率分别是38.29%、23.23%和95.57%。

Objective To study the constituents of Agelas aff. nemoechinata. Methods The isolation and purification of the compounds were performed by Sephadex LH-20, silica gel, ODS chromatography and HPLC methods. And their structures were determined by comparison their physicochemical characters and spectral data with literatures. Results From the methanol extracts , eight compounds were isolated and identified as Uracil （1）, Thymine （2）, 1H-2-pyridone （3）, 2-deoxythymidine （4）, 2-pyrrole-carboxamide（5）, 1H-indole-3-carbaldehyde（6）, 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofu- ran-2（4H）-one（7） and 5-aminopentanoic acid （8）. Conclusion Compounds 1-8 were isolated from this sponge for the first time. Compound 7 exhibited moderate cytotoxicity against K562, P388 and HeLa tumor cell lines with the proliferation inhibition rate 38. 29%, 23. 23% and 95.57%, at the concentra- tion level of 50 μg/mL, respectively.