土鳖虫含药血清对鸡胚绒毛尿囊膜血管生成的影响

Effect of Tubiechong-Containing Serum on Angiogenesis in Chick Chorioallantoic Membrane

目的探讨土鳖虫抗肿瘤的可能作用机制。方法将56枚鸡胚制备成鸡胚绒毛尿囊膜(CAM)模型,随机分为空白组和土鳖虫含药血清高、中、低剂量组;每组14枚。空白组不处理,土鳖虫含药血清高、中、低剂量组分别加入经灌胃含土鳖虫生药11g/(kg·d)、8g/(kg·d)、5.5g/(kg·d)剂量取得的大鼠土鳖虫含药血清,继续孵育72h后,观察各药物组鸡胚的存活情况、CAM模型的形态变化、CAM模型血管生成数量。结果加药72h后,各组鸡胚存活率相当。存活鸡胚可见胚胎活动活跃,CAM透明,分布清晰且血管颜色鲜红。死亡鸡胚CAM血管颜色变浅,脉络不清,胚胎活动消失。土鳖虫含药血清高、中、低剂量组新生血管数量均少于空白组(P<0.05);土鳖虫含药血清高剂量组新生血管数量少于土鳖虫含药血清中、低剂量组(P<0.05);而土鳖虫含药血清中、低剂量组间新生血管数量比较差异无统计学意义(P>0.05)。结论土鳖虫抗肿瘤作用可能与其抑制CAM血管生成有关,且大剂量效果更佳。

Objective To research the possible anti-neoplastic mechanism of Tubiechong （Eupolyphaga Seu Steleophaga）. Methods Totally 56 chick embryos were prepared into the chick chorioallantoic membrane （CAM） models and randomized into the blank group and Tubiechong-containing serum high-dose, middle-dose and low-dose groups. The blank group was given no treatment. The Tubiechong-containing serum high-dose, middle-dose and low- dose groups were given 11 g/kg·d, 8 g/kg·d and 5.5 g/kg·d Tubiechong-containing serum respectively. The in- cubation was continued for 72 hours. The survival of chick embryos, morphology of CAM models and angiogenesis of CAM models were observed Results The survival rate of chick embryos in each group was same. The embryo was active, CAM was transparent and vascular was clear and red in survival chick. The vascular was lighter and unclear and embryo was inactive in the dead chick. Comparing with the blank group, the angiogenesis in the Tubiechong-containing serum high-dose, middle-dose and low-dose groups were significantly decreased （P 〈 0. 05 ）. Comparing with the Tubiechong-containing serum middle-dose and low-dose groups, the angiogenesis in the Tubiechong-containing serum high-dose group was significantly decreased （ P 〈 0. 05 ）. There was no significant difference between the Tubiechong- containing serum middle-dose and low-dose groups in the angiogenesis （P 〉 0. 05）. Conclusion The anti-neoplastic effect of Tubiechong may be related to inhibiting the angiogenesis of CAM, with a better effect of large dose.