液相色谱-串联质谱法测定浓缩樱桃李汁中罗丹明B和苏丹红染料

Determination of Rhodamine B and Sudan Dyes in Myrobalan Plum Juice Concentrate by High Performance Liquid Chromatography-tandem Mass Spectrometry Mass Spectrometry

建立了液相色谱-串联质谱（HPLC-MS/MS）法同时检测浓缩樱桃李汁中罗丹明B和苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ5种染料的分析方法。样品经水稀释、乙腈提取,凝胶渗透色谱（GPC）净化,采用Zorbax Eclipse Plus C18色谱柱进行分离。在电喷雾正离子模式下（ESI＋）,用多重反应监测（MRM）方式进行离子监测,基质匹配外标法定量。5种染料在各自线性范围内,相关系数（r^2）均大于0.998,检出限（信噪比=3）为0.2～3.0μg/kg,定量限（信噪比=10）为0.5～10.0μg/kg,回收率在74.4%～95.3%之间,相对标准偏差为3.6%～7.9%。

A new method was developed for the simultaneous determination of Rhodamine B and Sudan Ⅰ、Ⅱ、Ⅲ、Ⅳin Myrobalan Plum juice concentrate using high performance liquid chromatography-tandem mass spectrometry. The sample was diluted by water and extracted with acetonitrile,then cleaned up by gel permeation chromatography. The analytes were separated on a Zorbax Eclipse Plus C18 column（100 × 3.0 mm, 1. 8 μm）. Electrospray positive ion source, multiple reaction monitoring mode, black matrix external standard method were used to analyse samples. The good correlation coefficients（r^2〉0. 998） of five dyes were obtained within their respective linear ranges. The limits of detection were in the range of 0.2～3.0μg/kg（S/N=3） ,and the limits of quantification were in the range of 0.5-10.0 μg/kg（S/N= 10）. The recoveries were in the range of 74.4%-95.3% with the relative standard deviations varying from 3.6%-7.9%.