苯乙烯不对称环氧化反应液的高效液相色谱法分析

Analysis of the Asymmetric Epoxidation Reaction Liquid of Styrene by HPLC

建立了Salen-Mn（Ⅲ）催化苯乙烯不对称环氧化反应的手性高效液相色谱分析法。采用手性AD-H柱,以正己烷和异丙醇为流动相,207nm作为检测波长,成功分离测定了苯乙烯及其环氧化产物环氧苯乙烷对映异构体。通过实验求得苯乙烯及（R）/（S）-环氧苯乙烷的线性回归方程,其相关系数分别为0.9985、0.9997、0.9995;方法检测限分别为0.02、0.10和0.13μg/mL;该方法低、中、高三个浓度水平的回收率为100.2%~100.8%;相对标准偏差分别为0.86%、1.52%和1.80%。

A chiral HPLC method was developed for the study of asymmetric epoxidation of styrene catalyzed by Salen-Mn（ Ⅲ ）. The baseline separation of styrene and（R）/（S）-styrene oxide enantiomers was achieved on a chiral AD-H column with n-hexane and isopropanol（99： 1） as the mobile phase at 207 nm. The correlation coefficients were 0. 9985,0. 9997 and 0. 9995 obtained from the linear regression equations of styrene and（R）/（S）-styrene, respectively. The detection limits of styrene and（R）/（S）-styrene were 0.02,0.10 and 0.13μg/mL, respectively. The recoveries of three levels were in the range from 100.2% to 100.8%. And the relative standard deviations were 0.86%, 1.52 G and 1.80%, respectively.