摘要
目的 观察人肺癌组织中黑皮素受体4(MC4R)基因的表达.方法 应用逆转录-聚合酶链反应(RT-PCR)方法检测15例肺癌及其癌旁组织中MC4R mRNA的相对表达量,并采用Western blot法检测上述组织中MC4R蛋白表达.结果 80.0% (12/15)的肺癌组织中MC4R mRNA和蛋白表达上调,而癌旁组织中26.7% (4/15)该基因表达上调和蛋白表达增加,两者差异有统计学意义(P<0.05).结论 肺癌组织中MC4R表达处于上调状态,可能在肺癌的发生、发展过程中发挥重要作用.
Objective To observe the expression of mehmocortin 4 receptor (MC4R) gene in human lung cancer.Methods The samples were obtained from 15 cases with lung cancer specimens.Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) strategies and Western blot were used for analyzing the MC4R mRNA and protein expression in 15 samples and adjacent noncanerous tissues.Results MC4R mRNA and protein expression was increased in 80.0% of human lung cancer and in 26.7% of adjacent noncanerous tissues (P < 0.05).Conclusion MC4R activation might be in human lung cancer.MC4R gene may play some roles in the pathogenesis of human lung cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第8期1635-1636,共2页
Chinese Journal of Experimental Surgery
关键词
肺癌
黑皮素受体4
Lung cancer
Mehmocortin 4 receptor